to differentiate between agarose gel electrophoresis and SDS-PAGE based on the following criteria:
2.2.1 Composition of gels
2.2.2 Dye used for staining

Answers

Answer 1

Explanation:

Agarose has a large pore size and is suitable for separating nucleic acids and large protein complexes. ... SDS-PAGE separates proteins primarily by mass because the ionic detergent SDS denatures and binds to proteins to make them uniformly negatively charged.

Answer 2

In this question we will differentiate between agarose gel electrophoresis and SDS-PAGE based on the composition of gels and Dye used for staining.

Agarose gel

The gel matrix is ​​created by dissolving a natural polysaccharide called dye-free agarose, which is derived from a type of seaweed, in a conductive buffer, in the approximate proportion of 1% to 3% agarose.

SDS-PAGE

Polyacrylamide gel electrophoresis (PAGE) with sodium dodecyl sulfate (SDS). SDS-PAGE consists of a type of electrophoresis performed under denaturing conditions for the separation of proteins without dyes.

Whit this information, we can conclude that differentiate between agarose gel electrophoresis and SDS-PAGE based on the composition of gels and Dye used for staining.

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Related Questions

17. Explain how carbon reservoirs can
be accessed without the influence
of humans.

Answers

Answer:Without human interference, the carbon in fossil fuels would leak slowly into the atmosphere through volcanic activity over millions of years in the slow carbon cycle. ... About half of these emissions are removed by the fast carbon cycle each year, the rest remain in the atmosphere.

Explanation:Hope this helps you!

In the slow carbon cycle, volcanic activity would release fossil fuel carbon into the atmosphere over millions of years without human intervention. The rapid carbon cycle removes half of these emissions annually, leaving the rest in the atmosphere.

What are 4 carbon reservoirs?

These are the reservoirs or sinks that carbon moves through as it cycles through the environment. Carbon is returned to the atmosphere through a wide number of different processes, including the decomposition of organisms, the eruption of volcanoes and fires, the combustion of fossil fuels, and more.

Carbon reservoirs contain substantial quantities of carbon between processes.

Burning fossil fuels such as natural gas, coal, and oil releases CO2, which is damaging to the environment and to individuals.
Using fossil fuels reduces carbon stores as well.
Toxic gases and smoke are released by industrial chimneys as a result of the combustion of fossil fuels.

Therefore, the carbon stocks such as fossil fuels have been used by humanity.

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The Cell Theory also states that all living cells have chloroplasts and a cell
wall.
True or false

Answers

Answer:

False

Explanation:

Cell walls are only found in plant cells.

As the water is forming into ice on tree branches are the molecules (gaining/losing) freedom of movement?

Answers

answer: losing

as the the water begins to freeze so does the molecules

1. It takes 29.5 days for the Moon to orbit Earth. How many days and hours does it take
7 days 15 hours per quarter
0 7 days 10 hours per quarter
0 7 days per quarter
0 7 days and 5 hours per quarter

Answers

7 days per quarter that’s why we have 7 days in a week... that was totally bs I made it up


PLEASE HELP !! ILL GIVE BRAINLIEST *EXTRA 40 POINTS* DONT SKIP :(( .!

Answers

Answer:

D

Explanation:

All the other answers dont really make as much sense. So D is best answer.

:)

Need help asap explain how scientists can use spectra to determine substances on another planet is what it says at the bottom

Answers

You can use spectra to determine substances on another planet is what it says at the bottom. This explains how scientists can use spectra to determine.

Please select the word from the list that best fits the definition

Grouping items together to keep observations organized


inferring


predicting


classifying


observing

Answers

Answer:

classifying

Explanation:

for example like classifying the files from SVU classifying them keeps them more organized and labeled kind of like identifying them classifying them keeps them all together in one group

Why is transcription both the same and different between chloroplasts and mitochondria?

Answers

Answer:

because they have similar functions

1 pts
What happens in nuclear fission?
Energy is released when two atoms collide with one another.
O Energy is released when two atoms travel at the speed of light.
O Energy is released when two atoms are combined.
O Energy is released when an atom is split apart.

Answers

When the two atoms are split

A guiding question instead of a hypothesis is most likely used in which of the following?

A. Scientific investigation
B. Laboratory experiment
C. Laboratory test
D. Repeated trials​

Answers

The answer is C lmk if you have any questions

anyone know this I don’t get the question

Answers

Answer: 5.000/ml

Explanation: density = mass/volume= 50/ 100=1/2 or 0.5 g/ mL

. The average adult (like Hank) is _________% water, ________% protein, _______% fat, _______% minerals, and _______% carbohydrates. 2. All the macromolecules that make you up come from ______________________________. 3. __________________________ reactions destroy the reactants that you give them, making big, complex substances smaller and __________________________ reactions reassemble the smaller products into new, bigger products. The processes behind these two sets of reactions make up your

Answers

Answer:

1. 62 percent water, 16 percent fat, 16 percent protein, 6 percent minerals, and less than 1 percent carbohydrate, along with very small amounts of vitamins and other miscellaneous substances.

2. Food.

3. Catabolic, Anabolic, Metabolism

Jan drew a diagram to compare centipedes and millipedes. Which belongs in the area labeled Y?
A.are active predators
B.have two pairs of legs on each segment
C.use specialized mouthparts to attach to hosts
D.use claws to inject venom

Answers

Answer:

BBB millipedes have two sets of legs on each segment.

Explanation:

i took the test.

Answer:

B.have two pairs of legs on each segment

Explanation:

Please like and have a good day

The picture below shows the energy flow through a meadow community.

Hurry!!!

Which of the following would most likely occur to the populations of the community immediately after a wildfire burns the environment?

Group of answer choices

Hawks and foxes would increase

Hawks would increase and foxes would decrease

Meadow plants would increase and rabbits would decrease

Meadow plants and rabbits would decrease

Answers

I think meadow plants & rabbits would decrease. It would take awhile before the plants would regrow & the rabbits would lose their food supply from their habitat, which effects the rabbits population.

In North America, the eastern spotted skunk mates
in late winter, and the western spotted skunk mates
in late summer. Even though their geographic
ranges overlap, the species do not mate with each
other. What most likely prevents these two species
from interbreeding?
A. habitat isolation
B. gametic isolation
C. geographic isolation
D. reproductive isolation

Answers

The answer is gonna be B

Reproductive isolation is a mechanism through which species do not get to interbreed. Option D. reproductive isolation

What is reproductive isolation?

This is a mechanism through which different species or populations do get to mate and produce fertile offspring. This mechanism allows the accumulation of genetic differences and favors speciation.

There are different barriers that inhibit or interrupt the genetic flow between different species.

Reproductive barriers prevent mating between two or more species. There are two types of barriers,

prezygotic ⇒ that avoids fertilization between individuals of different species, postzygotic ⇒ that impedes the zygote to develope and reach the adult stage.

Pre-copulatory o pre-ziygotic:

• Ecological or by habitat isolation;

Seasonal or temporal;

• Séxual o ethologic;

• Mechanic;

• Gametes incompatibility.

Postzygotic mechanisms or barriers include

• Hybrid inviability,

• Hybrid sterility,

• Cytoplasmic interactions.

In the exposed example occurs a seasonal difference between the mating period of the eastern spotted skunk and the western spotted skunk.

Probably, if both of them would mate during the same season and in the same area, they could reproduce.

However, since their mating season differs, individuals from each species do not get to cross and hence, produce fertile offspring.

Option D. reproductive isolation is what most likely prevents these two species from interbreeding

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Describe sexual reproduction in your own words.

Answers

Answer:

Sexual reproduction is the process in which new organisms are created, by combining the genetic information from two individuals of different sexes. The genetic information is carried on chromosomes within the nucleus of specialized sex cells called gametes.

Explanation:

the starting cell must duplicate its chromosomes in interphase before mitosis or meiosis can begin. does this change the number of chromosomes? chromatoids? both? how many of each would you expect in a human cell after interphase? (i need the second question answered plz)

Answers

the link is in the file above

As the Earth's climate continues to get warmer, air quality is expected to get worse (e.g., ozone level and particulate matter).
Group of answer choices

True

False

Answers

The answer is True hope this helps

AG CLASS HELP PLZ!!!!!!!

Complete the following sentence.
A turf management professional seeking advancement may choose to get certification through a
____________-_____________ program offered by a university as part of its continuing education options.

sorry no options

Answers

Answer:

the government

Explanation:

because i am reading in 7 class

Bacteria that are resistant to more than one antibiotic are called superbugs. Compare the control and antibiotic data from the three farms and select all the true statements. A: Another superbug was created by using the same method of giving antibiotics to chickens as Monita Farms. B: Our experiment was a failure. VIC: The data for the control farm shows that not administering antibiotics helps to prevent superbug creation. D: Natural selection is not a possible cause of the superbug creation on Monita Farms. E: There was no reason to test the control solution.​

Answers

A and C, I got it correct

Answer: There was no reason to test the control solution.​

Explanation: Bacteria that are resistant to more than one antibiotic are called superbugs. Compare the control and antibiotic data from the three farms and select all the true statements. A: Another superbug was created by using the same method of giving antibiotics to chickens as Monita Farms. B: Our experiment was a failure. VIC: The data for the control farm shows that not administering antibiotics helps to prevent superbug creation. D: Natural selection is not a possible cause of the superbug creation on Monita Farms. E:

Importance of photosynthesis to humans

Answers

Green plants and trees use photosynthesis to make food from sunlight, carbon dioxide and water in the atmosphere: It is their primary source of energy. The importance of photosynthesis in our life is the oxygen it produces. Without photosynthesis there would be little to no oxygen on the planet.

Answer: Green plants and trees use photosynthesis to make food from sunlight, carbon dioxide and water in the atmosphere: It is their primary source of energy. The importance of photosynthesis in our life is the oxygen it produces. Without photosynthesis there would be little to no oxygen on the planet.

Explanation:

Imagine you are producing a short film depicting the molecules and biological processes involved in gene expression. For this, you need to first create a storyboard for your film. A storyboard is a sequence of drawings, along with some description or dialogue, that illustrates and represents the scenes of a story or movie. Create a storyboard for the process of gene expression/central dogma depicting the story of how the cell uses the information in DNA to synthesize a protein.

Answers

Answer:

By decoding information with the help of transfer RNA (tRNA).

Explanation:

The cell uses the information in DNA with the help of transfer RNA (tRNA) to synthesize a protein because transfer RNA (tRNA) decode a messenger RNA (mRNA) in order to produce proteins according to the information present in messenger RNA (mRNA). The information about protein is stored in a gene's DNA which is passed to a RNA in the cell nucleus which is responsible for formation of copies of DNA in the process of translation. So the cell uses the information of gene's DNA with the help of RNA molecules.

What is needed for evolution to take place?

Answers

Answer:

genetic variation

Explanation:

GIVING 20 POINTS PLEASE HELP ME ASAP!!!!!

Answers

Answer:

The Answer is gonna be D. There are fewer winds in summer to interfere with drying up puddles

It’s D or E. They both show reasons the water could’ve left during the summer. But I strongly think it’s D.

Which type of mining is likely the least harmful to the environment?

Answers

Answer:

Placer mining can be less damaging to the environment than surface mines, as the sediment is returned to the water after the minerals have been extracted.

Explanation:

wikang pambansa noon at ngayon​

Answers

Filipino
I guess hehe

Use the data you calculated in parts A and B to explain why gypsy moths could threaten biodiversity in the ecosystem.


Answer: edmentum

The number of gypsy moths is growing exponentially in a short period of time, which suggests that there are few predators controlling their population. This growth also aids the exponential destruction of shrubs and trees, which native species rely on for food and shelter. Increased competition for these resources will likely cause the population of many native species to decline. Over time, some of the native species may die out or leave the area, leading to a decline in biodiversity.

Answers

Answer:

The number of moths are growing fast in just a short period of time, this means that their poulation is being controlled by their predators. As the population of the moths continue to grow, there will be an increase in the destruction of crops and trees within the moth's environment. Over time some of the species may die out and cause them to relocate and cause a decrease in biodiversity.

Explanation:

I put this answer in my own words, so you may copy and paste it.

The caterpillar of the Gypsy Moth provokes tree damage but since it is the only stage of the life cycle that utilizes food. When the population of feeding caterpillars reaches pandemic proportions, they can completely defoliate trees.

What is a biodiversity?

The term biodiversity pertains to the diversity of life on Earth at all levels, from genes to ecosystems, and can encompass the evolutionary, ecological, and cultural processes that enable life to exist.

The caterpillar of the Gypsy Moth provokes tree damage but since it is the only stage of the life cycle that utilizes food.

When the population of feeding caterpillars reaches pandemic proportions, they can completely defoliate trees.

Thus, this way gypsy moths could threaten biodiversity in the ecosystem.

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Is a volcano part of the lithosphere? Explain. NO WEIRD LINKS!

Answers

Answer: Tectonic activity is responsible for some of Earth's most dramatic geologic events: earthquakes, volcanoes, orogeny (mountain-building), and deep ocean trenches can all be formed by tectonic activity in the lithosphere.

Please give me brainlest if this helps.

Hemophilia is
A. an autosomal dominant inherritated disorder
B. an autosomal recessive inherited disorder
C. an Y- linked recessive disorder
D. an X- linked recessive inherited disorder ​

Answers

the answer is C jsjsjs

Help please!! Louisa wants to find out whether earthworms prefer to live in the dark or in the light. She constructs an investigation. She fills a box with dirt and places 30 earthworms in the dirt. Then, she covers half of the box with a lid. She leaves the other half open. After waiting a week, what should Louisa do next? No links!!!

A. repeat the experiment
B. write a report to present to her science class
C. ask another question about the behavior of earthworms
D. count the number of earthworms that are present on each side of the box

Answers

Answer:

A is the answer because she have to answer again to make sure it is right

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